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Effects of corn steep liquor on production of 2,3-butanediol and acetoin by Bacillus subtilis

Paper ID Volume ID Publish Year Pages File Format Full-Text
34591 45035 2013 8 PDF Available
Title
Effects of corn steep liquor on production of 2,3-butanediol and acetoin by Bacillus subtilis
Abstract

•Acetoin was a main ingredient under low corn steep liquor (CSL) levels.•2,3-BD became a major product under high CSL levels.•Overexpression of acetoin reductase reduced CSL supplement for 2,3-BD production.•CSL enhanced acetoin production with acr::cat mutant strain.

The initial concentration of corn steep liquor (CSL) have remarkable effects on not only 2,3-butanediol (2,3-BD) and acetoin (metabolic precursor) production, but also on the ratio of 2,3-BD to acetoin. When a high concentration of CSL was supplemented, cell growth was improved, acetoin reductase (ACR) was stimulated, the concentration of 2,3-BD increased by 78.6%, acetoin decreased by 61.9%, and the ratio of 2,3-BD to acetoin increased by 3.69-fold. The acr gene, encoding ACR, was over-expressed in Bacillus subtilis. Compared to the control (parent strain), low levels of CSL in the engineered strain increased 2,3-BD concentration and the ratio 2,3-BD to acetoin by 13.9% and 39.5%, respectively, and decreased acetoin titer by 18.3%. Acetoin became a major product under low levels of CSL. Also, a knockout strain carrying an acr::cat insertion mutation was constructed. As expected, the loss of ACR activity led to an accumulation of acetoin in the supernatants of acr:: cat mutant cultures. Additionally, the productivity of acetoin was improved by high concentration of CSL. The results above demonstrate the feasibility of using B. subtilis for the production of not only 2,3-BD but also acetoin as a major product.

Keywords
2,3-Butanediol; Acetoin; Corn steep liquor; Acetoin reductase; Bacillus subtilis
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Effects of corn steep liquor on production of 2,3-butanediol and acetoin by Bacillus subtilis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1610–1617
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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