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Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
34593 45035 2013 9 PDF Available
Title
Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems
Abstract

•Comprehensive analysis of factors affecting the extraction of PHA monomers.•HA extraction differed between floccular and granular mixed cultures.•Larger and more substituted monomers have slower extraction kinetics.•Modified HA extraction procedures are proposed for different systems.•HA extraction impacts the assessment of PHA biopolymer quantity and quality.

For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.

Keywords
Polyhydroxybutyrate (PHB); Polyhydroxyvalerate (PHV); Polyhydroxy-2-methylbutyrate (PH2MB); Polyhydroxy-2-methylvalerate (PH2MV); Extraction and recovery
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Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1626–1634
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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