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In situ positron emission tomography monitoring of endothelial cells embedded in perfused fibrin gels

Paper ID Volume ID Publish Year Pages File Format Full-Text
34596 45035 2013 6 PDF Available
Title
In situ positron emission tomography monitoring of endothelial cells embedded in perfused fibrin gels
Abstract

•A PET imaging protocol for perfused 3D cell cultures has been devised.•PET imaging allowed repeated thick 3D culture monitoring over long periods.•3D culture sample perfusion, viability and metabolism could be assessed with 18FDG.•We tested the potential of PET imaging as a monitoring tool in tissue engineering.•We examined PET imaging as an instrument for perfused bioreactor culture monitoring.

In tissue engineering, the continuous monitoring of cell and tissue cultures in vitro is crucial to assess their functional status over time. However, these constructs can be large, thick and non-transparent. Medical imaging techniques can allow real-time in situ monitoring of cell and tissue cultures in thick solid scaffolds. Here, human endothelial cells were embedded in fibrin gels that were continuously perfused by a culture medium. Positron emission tomography (PET) imaging was used to assess cell viability non-destructively over periods extending up to a few weeks. PET imaging protocols were adapted and validated to measure culture perfusion and cell metabolism using [18F]-fluorodeoxyglucose (18FDG). Cell densities down to 100,000 cells/mL were detectable after 12 h of culture and cell structures were localized within the fibrin gels after 1–2 weeks of culture. PET is a promising tool to investigate a wide range of cellular properties and reveal information on tissue development.

Keywords
Dynamic cell culture; Positron emission tomography (PET); Endothelial cells; Tissue engineering; [18F]-fluorodeoxyglucose (18FDG) uptake
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In situ positron emission tomography monitoring of endothelial cells embedded in perfused fibrin gels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1645–1650
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us