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Interactions of bovine serum albumin with biological buffers, TES, TAPS, and TAPSO in aqueous solutions

Paper ID Volume ID Publish Year Pages File Format Full-Text
34601 45035 2013 11 PDF Available
Title
Interactions of bovine serum albumin with biological buffers, TES, TAPS, and TAPSO in aqueous solutions
Abstract

•The thermal stability of BSA protein in aqueous buffer solutions was investigated.•The biological buffers used in this study are TES, TAPS, and TAPSO.•The studied buffers can be used as a potential stabilizer for proteins.•DLS, UV–vis, fluorescence, and molecular docking techniques were used in this study.

The thermal stability of bovine serum albumin (BSA) in the aqueous solutions containing the biological buffers, N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES), N-[tris(hydroxylmethyl)methyl]-3-aminopropanesulfonic acid (TAPS), and N-[tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid (TAPSO), was studied by using dynamic light scattering (DLS) at various temperatures and concentration ranges of buffers. It is found that the increase of the buffer concentration enhanced the thermal stability of protein BSA, and the stabilization tendency follows the order of TAPSO > TES ≈ TAPS. In this study, we have also investigated the interactions of BSA with TES, TAPS, and TAPSO by using various techniques, such as UV–vis absorption, fluorescence, and molecular docking. It is revealed that the main interactions between the studied buffers and the peptide moieties of proteins are electrostatic including hydrogen bonds. The results obtained from this series of studies confirmed that the biological buffers, TES, TAPS, and TAPSO can serve as good stabilizers for the globular protein BSA, in the aqueous solutions.

Keywords
Protein stability; Molecular Docking; Buffers; BSA; Spectroscopy
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Interactions of bovine serum albumin with biological buffers, TES, TAPS, and TAPSO in aqueous solutions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1686–1696
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us