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Diversity and analysis of sequences encoded by arcelin genes from Indian wild pulses resistant to bruchids

Paper ID Volume ID Publish Year Pages File Format Full-Text
34602 45035 2013 9 PDF Available
Title
Diversity and analysis of sequences encoded by arcelin genes from Indian wild pulses resistant to bruchids
Abstract

•Insecticidal arcelin from various wild pulses was amplified, sequenced and deduced.•They differ in their amino acid sequences and various physico-chemical properties.•Secondary structures docked with various sugars showed its affinity to glucosamine.•It is expected to offer prospective factor against insect pests to crop protection.

Wild pulse accessions are considered a vital source of genes for insect resistance for crop improvement programmes. Wild pulses resistant to infestation towards the bruchid insect pest, Callosobruchus maculatus from South India were chosen to screen the existence of potent insecticidal protein, arcelin from APA locus (Arcelin/Phytohemagglutinin/α-Amylase inhibitor) to ascertain their nature and functional diversity without any specific indication for insect resistant factors. The DNA sequence coding for arcelin from various species of wild pulses were amplified, sequenced and deduced to their protein sequences. These protein sequences were examined physico-chemically using several bioinformatics tools and docked with various sugars to resolve the nature of arcelin molecules. Results indicated the presence of significant differences in the properties of arcelin molecules from various species of Indian wild pulses with their amino acid sequences, several physico-chemical properties and binding ability with sugars. The differences observed on these arcelin molecules from diverse wild pulses are predicted to provide a prospective insect pest control factors.

Keywords
Insecticidal protein; Arcelin; Wild pulses; Molecular docking; Bruchids
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Diversity and analysis of sequences encoded by arcelin genes from Indian wild pulses resistant to bruchids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1697–1705
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us