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Cellobiose dehydrogenase from the ligninolytic basidiomycete Phlebia lindtneri

Paper ID Volume ID Publish Year Pages File Format Full-Text
34604 45035 2013 9 PDF Available
Title
Cellobiose dehydrogenase from the ligninolytic basidiomycete Phlebia lindtneri
Abstract

•The first purified cellobiose dehydrogenase in genus Phlebia.•Complete characterization of CDH comprising kinetics, pH and temperature dependence.•The first gene and cDNA sequence of cellobiose dehydrogenase in genus Phlebia.

Cellobiose dehydrogenase (CDH), an extracellular flavocytochrome produced by several wood-degrading fungi, was detected in the culture supernatant of the selective delignifier Phlebia lindtneri maintained on a cellulose-based liquid medium. Cellobiose dehydrogenase was purified to homogeneity by a rapid procedure, using ammonium sulfate precipitation, ion-exchange chromatography, and chromatofocusing. The enzyme was recovered with a 61.2 fold increased specific activity and a yield of 47.5%. As determined by SDS-PAGE, the molecular mass of the purified enzyme was found to be 104.5 kDa and its isoelectric point was 4.0. The carbohydrate content of the purified enzymes was 22%. In this work, the cellobiose dehydrogenase gene cdh1 and its corresponding cDNA from fungi Phlebia lidnteri were isolated, cloned, and characterized. The 2319 bp full-length cDNA of cdh1 encoded a mature CDH protein containing 755 amino acids, which was preceded by a signal peptide of 17 amino acids. The deduced protein sequence of cdh1 shared significant similarity with other known fungal cellobiose dehydrogenase.

Keywords
Cellobiose dehydrogenase; Phlebia; Gene; Fungi
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Cellobiose dehydrogenase from the ligninolytic basidiomycete Phlebia lindtneri
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 11, November 2013, Pages 1715–1723
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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