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Optimization of biosurfactant production using waste from biodiesel industry in a new membrane assisted bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
34619 45037 2013 8 PDF Available
Title
Optimization of biosurfactant production using waste from biodiesel industry in a new membrane assisted bioreactor
Abstract

•Optimized rhamnolipids production using crude glycerol (CG) from biodiesel waste.•Rhamnolipids production using CG was about four times higher than previously reported.•Crude glycerol had little effect on rhamnolipids composition and properties.

The main byproduct of biodiesel production is glycerol. Here, crude glycerol – byproduct of biodiesel industry – was evaluated as sole carbon source in rhamnolipids production by Pseudomonas aeruginosa. The optimal concentration of crude glycerol and sodium nitrate was assessed using response surface methodology, resulting in about 40–50 mg/L.h of rhamnolipids, which was about four times higher than previously reported in the literature. Fermentation parameters were similar to those observed with commercial glycerol as sole carbon source. The optimized medium was suitable for production using simple (22.9 mg/L.h) and fed-batch (32.4 mg/L.h) fermentation in oxygen-controlled bioreactor without foaming formation. Composition and relative abundance of rhamnolipid congeners showed that crude glycerol had little effect on metabolic pathways involved in their production. CMC values were approximately 130 mg/L and 230–260 mg/L for rhamnolipids from crude and commercial glycerol fermentation, respectively, which were about 2–6 times lower than CMC values of synthetic surfactants.

Keywords
Factorial design; Surface response analysis; Rhamnolipid; P. aeruginosa; Bioreactor; Crude glycerol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 9, September 2013, Pages 1271–1278
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us