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A simple process for the isolation of epithelial cells from bacteria-contaminated samples using anchoring molecules

Paper ID Volume ID Publish Year Pages File Format Full-Text
34640 45037 2013 7 PDF Available
Title
A simple process for the isolation of epithelial cells from bacteria-contaminated samples using anchoring molecules
Abstract

•Epithelial cells were isolated from bacteria contaminated sample.•No biological materials were utilized to isolate epithelial cells.•20 min were required to achieve bacteria free epithelial cells.

A simple and efficient tool to isolate epithelial cells from bacteria-contaminated samples has been developed using two different microparticles functionalized with chemical molecules. The epithelial cells could be captured simply by biocompatible anchors for membranes (BAM), consisting of poly(ethylene glycol) functionalized with oleyl-chain-conjugated NHS (N-hydroxysuccinimide) on glass microparticles, whereas bacteria were adsorbed on 3-aminopropyltrimethoxysilane (ATPS)-functionalized magnetic microparticles. In the case of samples highly contaminated with bacteria, epithelial cells were not isolated successfully by both of the single BAM- and antibody-functionalized microparticles. Therefore, serial isolation steps of these two different chemical functionalized microparticles were introduced. The concentration of bacteria was decreased dramatically by using APTS-functionalized magnetic particles prior to the isolation of epithelial cells by BAM microparticles. With these serial processes, successful isolation of epithelial cells was achieved from bacteria-contaminated epithelial samples. The applicability of this method was verified with bacteria-contaminated intestinal samples biopsied from a BALB/C mouse for primary cell cultivation.

Keywords
Epithelial cell isolation; Bacteria removal; Anchoring molecule; BAM; Physical adsorption
First Page Preview
A simple process for the isolation of epithelial cells from bacteria-contaminated samples using anchoring molecules
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 9, September 2013, Pages 1429–1435
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering