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An air-lift biofilm reactor for the production of γ-decalactones by Yarrowia lipolytica

Paper ID Volume ID Publish Year Pages File Format Full-Text
34644 45038 2014 6 PDF Available
Title
An air-lift biofilm reactor for the production of γ-decalactones by Yarrowia lipolytica
Abstract

•New simple way to immobilise yeast for an improved production of lactones.•Y. lipolytica in biofilm presents specific morphological and metabolic characteristics.•Aeration triggers yeast hydrophobic surface properties and adhesion to plastic.•Detection of yeast subpopulation of importance to biotransformation.

Decalactones are interesting flavouring compounds that can be produced from ricinoleic acid. In this study, the production of lactones in biofilms using Yarrowia lipolytica is investigated. The hydrophobia of cells increased for increased aeration rates resulting in higher adhesion when the reactor wall was hydrophobic (plastic). To increase adhesion, sheets of methyl-polymethacrylate (PMMA) were added in the reactor and the production of lactones increased with the surface of plastic added, reaching 850 mg/L of 3-hydroxy-γ-decalactone for 60 cm2. In an Airlift bioreactor made of PMMA, biofilms were present at the top of the reactor for increased aeration. In the meantime, a metabolic shift occurred resulting in high amounts of 3-hydroxy-γ-decalactone. At 0.493 vvm and 61 h of culture, the dissolved oxygen ratio was of 28.6% and cells grew to only 1.29 × 106 cells/mL in the liquid medium but 3-hydroxy-γ-decalactone accumulated to 1.7 g/L instead of less than 0.3 mg/L for lower aeration. Adhering cells had a particular elongated shape intermediate between the yeast and the pseudofilamentous forms. It is concluded that adhering Y. lipolytica cells are in a specific physiological state changing their structure but also their metabolic properties and these properties make them good candidate for simple immobilisation process.

Keywords
Yarrowia lipolytica; Lipid biotransformation; β-Oxidation; Surface properties; Aroma production; Biofilm reactor
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An air-lift biofilm reactor for the production of γ-decalactones by Yarrowia lipolytica
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1377–1382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us