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A microbial biosensor for hydrogen sulfide monitoring based on potentiometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
34646 45038 2014 9 PDF Available
Title
A microbial biosensor for hydrogen sulfide monitoring based on potentiometry
Abstract

•Thiobacillus thioparus was used in biosensing system for hydrogen sulfide detection.•Response time and calibration were calculated by variations of solution potential.•Potential alterations at surface adsorption method were more than other methods.•Response time of encapsulation and adsorption methods were measured at 160 s and 105 s, respectively.

In this study, a microbial biosensor for hydrogen sulfide (H2S) detection based on Thiobacillus thioparus immobilized in a gelatin matrix was developed. The T. thioparus was immobilized via either surface adsorption on the gelatin matrix or entrapment in the matrix. The bacterial and gelatin concentration in the support were then varied in order to optimize the sensor response time and detection limit for both methods. The optimization was conducted by a statistical analysis of the measured biosensor response with various bacterial and polymer concentrations. According to our experiments with both immobilization methods, the optimized conditions for the entrapment method were found to be a gelatin concentration of 1% and an optical density of 82. For the surface adsorption method, 0.6% gelatin and an optical density of 88 were selected as the optimal conditions. A statistical model was developed based on the extent of the biosensor response in both methods of immobilization. The maximum change in the potential of the solution was 23.16 mV for the entrapment method and 34.34 mV for the surface absorption method. The response times for the entrapment and adsorption methods were 160 s and 105 s, respectively. The adsorption method is more advantageous for the development of a gas biosensor due to its shorter response time.

Keywords
Potentiometry; Immobilization; Thiobacillus thioparus; Entrapment; Surface adsorption; Biosensor
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A microbial biosensor for hydrogen sulfide monitoring based on potentiometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1393–1401
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us