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Production of polyhydroxyalkanoates using hydrolysate of spent coffee grounds

Paper ID Volume ID Publish Year Pages File Format Full-Text
34648 45038 2014 6 PDF Available
Title
Production of polyhydroxyalkanoates using hydrolysate of spent coffee grounds
Abstract

•Hydrolysate of spent coffee grounds may be utilized for PHA production.•Burholderia cepacia is capable for P(HB-co-HV) copolymer biosynthesis.•Extraction of polyphenols prior to hydrolysis enhances PHA yields.

Spent coffee grounds (SCG) are solid fraction wastes deriving from coffee industries, the disposal of which represents a serious environmental issue. This work aims at the conversion of hydrolysate of SCG (SCGH) into polyhydroxyalkanoates (PHA) by Burkholderia cepacia. The bacteria was capable of SCGH utilization and production of copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate [P(HB-co-HV]. Levulinic acid present in SCGH probably served as the precursor of 3HV for the copolymer biosynthesis. To improve the PHA yields, various detoxification methods were tested. The extraction of polyphenols from SCG by ethanol prior to the hydrolysis seems to be the most promising, since, apart from the fact that it enhanced the PHA yields by about 25%, polyphenols extracted from SCG may represent important side products, because they might be used for the production of functional foods and other high value products.

Keywords
Spent coffee grounds; Polyhydroxyalkanoates; PHA; Burkholderia cepacia; Detoxification
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Production of polyhydroxyalkanoates using hydrolysate of spent coffee grounds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1409–1414
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us