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Chemical modification of chloroperoxidase for enhanced stability and activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
34656 45038 2014 8 PDF Available
Title
Chemical modification of chloroperoxidase for enhanced stability and activity
Abstract

•Chloroperoxidase from Caldariomyces fumago was chemically modified by 6 methods.•By using modified CPOs higher yields of Cbz-glycinal were achieved.•CPOs modified by carbodiimide and periodate were more stable at higher pH values.•CPOs modified by carbodiimide and periodate had improved thermostability.•Higher Cbz-glycinal yields at pH 7.0 and 50 °C were reached by using modified CPOs.

Chloroperoxidase from Caldariomyces fumago (CPO, EC 1.11.1.10) is one of the most interesting enzymes from the group of heme peroxidases and has been extensively applied in synthetic processes. Nevertheless, the practical application of CPO is limited due to its very low operational stability, especially in the presence of peroxidative compounds. For this reason, effect of chemical modifications of CPO in the stability of the enzyme was studied. Side-chain selective modifications of amino groups of Lys residues, and carboxyl groups of Asp and Glu residues, as well as crosslinking and periodate oxidation of sugar moiety were carried out. The stability of modified CPOs was evaluated at elevated pH and temperature, and in the presence of tert-butyl hydroperoxide. Effect of modification of CPO on the performance of the reaction of Cbz-ethanolamine oxidation was studied as well. Those modifications that involved carboxyl groups via carbodiimide coupled method and the periodate oxidation of the sugar moiety produced better catalysts than native CPO in terms of stability and activity at elevated pH values and temperatures.

Keywords
Chemical modifications; Chloroperoxidase from Caldariomyces fumago; Cbz-ethanolamine oxidation; Cbz-glycinal synthesis; Peroxide dependent inactivation
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Chemical modification of chloroperoxidase for enhanced stability and activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1472–1479
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us