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Improved catalytic efficiency of catalase from Bacillus subtilis by rational mutation of Lys114

Paper ID Volume ID Publish Year Pages File Format Full-Text
34659 45038 2014 6 PDF Available
Title
Improved catalytic efficiency of catalase from Bacillus subtilis by rational mutation of Lys114
Abstract

•The PoPMuSiC algorithm was applied for rational engineering of the Bacillus catalase.•Lys114 was mutated to increase the catalytic efficiency.•The variant Lys114Tyr showed great potential in the production of industrial catalase.

Alkaline catalases with good properties are desirable in the textile industry. In the present work, by applying the PoPMuSiC algorithm for the calculation of the folding free energy, Lys114 of a Bacillus catalase was rationally selected and engineered to improve the thermostability. Interestingly, the Lys114Tyr, Lys114Val, Lys114Met and Lys114Ile variants showed higher catalytic efficiencies when compared with the wild-type protein. In particular, the Lys114Tyr variant showed the highest catalytic efficiency, which was 5.3-fold of the wild-type catalase. The production of the Lys114Tyr variant may represent an improved catalase suitable for industrial purposes.

Keywords
Catalase; Enzyme engineering; Site-directed mutagenesis; Catalytic efficiency
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Improved catalytic efficiency of catalase from Bacillus subtilis by rational mutation of Lys114
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1497–1502
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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