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Characterization of enzyme-catalysed endogenous β-hydroxylation of phenylethanoid glycosides in Euphrasia rostkoviana Hayne at the molecular level

Paper ID Volume ID Publish Year Pages File Format Full-Text
34664 45038 2014 5 PDF Available
Title
Characterization of enzyme-catalysed endogenous β-hydroxylation of phenylethanoid glycosides in Euphrasia rostkoviana Hayne at the molecular level
Abstract

•Phenylethanoid glycosides in different extracts of Euphrasia rostkoviana were identified and quantified.•Acteoside and eukovoside were identified in intact samples.•Campneoside II and β-hydroxyeukovoside were identified in enzyme treated samples.•We proved for the first time that a β-hydroxylase enzyme is active in eyebright tissues.•The enzyme-hydrolyzed extracts are a good source of isolation of β-hydroxyl phenylethanoid glycosides.

Phenylethanoid glycosides, the main constituents of the aerial part of eyebright (Euphrasia rostkoviana Hayne) were treated by the endogenous hydroxylase enzyme and the concomitant biotransformation was characterized by applying high-performance liquid chromatography with UV and MS detections and NMR spectroscopy. In the extracts of the untreated (intact) samples, acteoside and eukovoside were determined as main compounds. The enzymatic treatment resulted in the quantitative transformation of these phenylethanoid glycosides into their corresponding hydroxyl derivatives identified as two epimers of β-hydroxyacteoside and β-hydroxyeukovoside. As to the importance of this hydroxylation β-hydroxyeukovoside was identified as a new compound and β-hydroxyacteoside was described for the first time in eyebright. We proved for first time that a β-hydroxylase enzyme is active in eyebright tissues which can transform phenylethanoid glycosides into their β-hydroxyl derivatives. Our new enzymatic method combined with a preparative HPLC facilitates the isolation of β-hydroxyl phenylethanoid glycosides from the aerial part of eyebright.

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Keywords
Verbascozide; Euphrasia officinalis; HPLC–MS; Endogenous enzymatic hydroxylation; Phenylethanoid glycoside
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Characterization of enzyme-catalysed endogenous β-hydroxylation of phenylethanoid glycosides in Euphrasia rostkoviana Hayne at the molecular level
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1533–1537
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us