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Purification and evaluation of a novel antioxidant peptide from corn protein hydrolysate

Paper ID Volume ID Publish Year Pages File Format Full-Text
34668 45038 2014 8 PDF Available
Title
Purification and evaluation of a novel antioxidant peptide from corn protein hydrolysate
Abstract

•Extrusion and starch removal were used as pretreatment procedures of corn protein.•Hydrolysate from corn protein was prepared using Alcalase.•Corn protein antioxidant hydrolysate was purified sequentially by ultrafiltration, ion exchange, gel filtration and RP-HPLC.•Sequence of purified peptide was determined to be Gln-Gln-Pro-Gln-Pro-Trp.•Purified novel peptide showed potent abilities to eliminate free radicals.

In the present study, corn protein hydrolysate (CPH) with antioxidant activity was obtained by enzymatic hydrolysis. Corn gluten meal (CGM) was hydrolyzed using two proteases (Alcalase and Protamex) to produce the antioxidant peptide. Extrusion and starch removal of corn protein were used as pretreatment procedures before proteolysis. Hydrolysis by Alcalase has more remarkable digesting efficiency on corn protein than that by Protamex. Therefore, the hydrolysate catalyzed by Alcalase was fractionated by ultrafiltration, and peptide with the highest antioxidant activity was purified from <6 kDa molecular weight fraction. The amino acid sequence of the novel peptide was Gln-Gln-Pro-Gln-Pro-Trp as identified by a quadrupole time-of-flight mass spectrometer (Q-TOF2), with molecular weight of 782.34 Da, which was matched to γ-zein f (50–55). The new peptide was further synthesized by Fmoc solid-phase method. It showed scavenging activity against DPPH, ABTS, and hydroxyl free radicals in dose dependent manner with EC50 values of 0.95, 0.0112 and 4.43 mg/mL, respectively. It also exhibited notable reducing power of 0.54 at 2.0 mg/mL, but showed weaker Fe2+-chelating capacity with EC50 value of 6.27 mg/mL. These results suggest that the hexapeptide is a potential natural antioxidant that can be used as drug or functional food ingredient.

Keywords
Corn protein; Hydrolysate; Antioxidant activity; Hexapeptide
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Purification and evaluation of a novel antioxidant peptide from corn protein hydrolysate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 9, September 2014, Pages 1562–1569
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us