fulltext.study @t Gmail

Broadening the synthetic potential of disaccharide phosphorylases through enzyme engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
34671 45039 2012 7 PDF Available
Title
Broadening the synthetic potential of disaccharide phosphorylases through enzyme engineering
Abstract

In recent years, disaccharide phosphorylases have attracted increasing attention as promising biocatalysts for the production of glycosylated compounds. These enzymes make use of a glycosyl phosphate as donor substrate, which is much cheaper than the nucleotide-activated donors required by glycosyl transferases. Unfortunately, the number of available donor specificities is rather limited, and typically only allow the transfer of either a glucosyl or a galactosyl residue. In addition, most phosphorylases have a strong preference for carbohydrate acceptors, and can thus only be used for the synthesis of saccharide chains. The engineering of their substrate specificity thus is of significant value to broaden the range of products that can be obtained. Furthermore, the stability of some phosphorylases will also need to be improved to allow their commercial exploitation in a variety of industrial processes. In this review, several strategies for the engineering of these parameters are discussed and illustrated with some recent successes.

► Disaccharide phosphorylases are promising biocatalysts for glycosylation reactions. ► The number of specificities that can be found in nature is rather limited. ► Enzyme engineering allows to broaden the range of products that can be synthesized.

Keywords
Disaccharide phosphorylases; Glycosylation; Glycosyl phosphate; Biocatalysis; Enzyme engineering; High-throughput screening
First Page Preview
Broadening the synthetic potential of disaccharide phosphorylases through enzyme engineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 11–17
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us