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Immobilization to improve the properties of Pseudomonas fluorescens lipase for the kinetic resolution of 3-aryl-3-hydroxy esters

Paper ID Volume ID Publish Year Pages File Format Full-Text
34686 45039 2012 8 PDF Available
Title
Immobilization to improve the properties of Pseudomonas fluorescens lipase for the kinetic resolution of 3-aryl-3-hydroxy esters
Abstract

Lipase AK “Amano” 20 from Pseudomonas fluorescens (PFL) was immobilized using diverse immobilization techniques. The methods developed, especially the optimized sol–gel procedure, enabled the fine tuning of enzymatic activity and enantioselectivity for the kinetic resolution of racemic ethyl 3-aryl-3-hydroxypropanoates. The aryl moieties of the racemates include furan-2 and 3-yl, thiophen-2 and 3-yl, benzofuran-2-yl, benzo[b]thiophen-2-yl, as well as phenyl and 4-chloro- and 4-methoxyphenyl groups. The optimized PFL sol–gel preparation (encapsulation from the aqueous solution of PFL, sucrose and Celite in situ) was shown to be efficiently reusable in ten cycles and highly enantioselective with E > 200 to all other substrates except furan-2 and 3-yl and thiophen-2 and 3-yl substituted compounds with E 108–184.

► Lipase AK (PFL) was immobilized by adsorption, CLEA technology and sol–gel encapsulation. ► Long-term stability and reuse experiments indicated the sol–gel preparations highly usable. ► The presence of sucrose and Celite in sol–gel encapsulation gave a new catalyst with excellent properties. ► Immobilized enzymes were applied in the kinetic resolution of several 3-aryl-3-hydroxypropanotes.

Keywords
Kinetic resolution; Ethyl 3-aryl-3-hydroxy propanoates; Pseudomonas fluorescens lipase; Sol–gel entrapment; Cross-linked enzyme aggregates (CLEA), adsorption
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Immobilization to improve the properties of Pseudomonas fluorescens lipase for the kinetic resolution of 3-aryl-3-hydroxy esters
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 119–126
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us