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Decoupling of oxygen transfer and power dissipation for the study of the production of pristinamycins by Streptomyces pristinaespiralis in shaking flasks

Paper ID Volume ID Publish Year Pages File Format Full-Text
3469 172 2012 9 PDF Available
Title
Decoupling of oxygen transfer and power dissipation for the study of the production of pristinamycins by Streptomyces pristinaespiralis in shaking flasks
Abstract

Streptomyces pristinaespiralis is a filamentous bacterium used in the pharmaceutical industry for the production of pristinamycins. In previous works, it was shown that the occurrence of production and the antibiotics concentration could be related to gas–liquid transfer and power dissipation in shaking flasks. Nevertheless, in standard cultures, both mechanisms are coupled. It is then a difficult task to assign a precise physiological response to either oxygen transfer or power dissipation. The aim of the present study was to decouple the oxygen transfer coefficient (kLa) and the power dissipation per unit of volume (P/V) to study their respective impact on pristinamycin production. During cultures in flasks, the rotation diameter of the shaking table was changed to modify the kLa but not the power dissipation P/V. The influence of operating conditions with P/V ranging from 0.55 to 10.3 kW m−3 and kLa ranging from 30 to 490 h−1 have been determined on the microbial kinetics and also on the pellet diameters. The final biomass concentration and the release of antibiotics were then related to kLa, whereas the final pristinamycins concentrations, as well as the bacterial pellet diameter were mainly correlated to P/V independently of the kLa. The change in the pellet diameter could be the crucial parameter for the pristinamycins production as it might influence the nutriment transfer inside the pellets and the ratio of active cells in each pellet.

► Decoupling of kLa and power dissipation in shaking flasks. ► Respective influence of OTR and power dissipation on antibiotic production. ► The release of pristinamycins is related to the oxygen transfer. ► The final concentration of pristinamycins is impacted by the power dissipation.

Keywords
Streptomyces; Pristinamycins; Power dissipation; Gas–liquid mass transfer coefficient; Shaking flask
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Decoupling of oxygen transfer and power dissipation for the study of the production of pristinamycins by Streptomyces pristinaespiralis in shaking flasks
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 68, 15 October 2012, Pages 25–33
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us