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Application of a Burkholderia cepacia lipase-immobilized silica monolith micro-bioreactor to continuous-flow kinetic resolution for transesterification of (R, S)-1-phenylethanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
34690 45039 2012 4 PDF Available
Title
Application of a Burkholderia cepacia lipase-immobilized silica monolith micro-bioreactor to continuous-flow kinetic resolution for transesterification of (R, S)-1-phenylethanol
Abstract

Burkholderia cepacia lipase was immobilized in silicates forming from n-butyl-substituted precursors within a silica monolith from methyl-substituted precursors. The resultant preparation gave about 12 times higher rates of transesterification of (R, S)-1-phenylethanol with vinyl acetate and an approximately two-fold increase in the enantioselectivity toward (R)-1-phenylethanol, as compared to a non-immobilized counterpart. The highest enzymatic activity and enantioselectivity (reaching 250) were found at a low water activity of 0.11. The continuous-flow kinetic resolution of (R, S)-1-phenylethanol was successfully conducted using lipase-immobilized silica monolith micro-bioreactors with various inside diameters ranging from 0.25 to 1.6 mm. The reactor performance during continuous operation was consistent with the prediction from the batch reactor. A steady state conversion of 40% and enantiomeric excess more than 98% were maintained over a time period of 15 days.

► Lipase-immobilized silica monolith is prepared using two-step sol–gel method. ► Lipase immobilized in butyl-silicate displays high activity and enantioselectivity. ► Silica monolith micro-bioreactor gives similar performance to batch reactor. ► Continuous-flow kinetic resolution is achieved stably with 98% enantiomeric excess.

Keywords
Kinetic resolution; 1-Phenylethanol; Burkholderia cepacia lipase; Sol–gel immobilization; Silica monolith; Micro-bioreactor
First Page Preview
Application of a Burkholderia cepacia lipase-immobilized silica monolith micro-bioreactor to continuous-flow kinetic resolution for transesterification of (R, S)-1-phenylethanol
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 147–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering