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High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase

Paper ID Volume ID Publish Year Pages File Format Full-Text
34691 45039 2012 5 PDF Available
Title
High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase
Abstract

Inulinase is an important enzyme used in the high fructose syrup and other related industries. A more cost-effective approach is required for producing highly active inulinase. In this study, the gene encoding inulinase of the yeast Kluyveromyces marxianus CBS 6556 was expressed in methylotrophic host Pichia pastoris and secretory production of recombinant inulinase (rKmINU) in the yeast under methanol induction was achieved. The purified rKmINU showed a specific activity of 2714 U/mg, which is over 12-fold higher than those of other inulinases described previously. It displayed excellent stability from 30 to 50 °C and pH 3.0–5.0, and the half-life of rKmINU was over 96 h under these conditions. Moreover, rKmINU saccharified Jerusalem artichoke tuber juice effectively.

► Secretory production of the rKmINU. ► Specific activity of the rKmINU over 12-fold higher than other inulinase. ► Half-life of the rKmINU at 50 °C and pH 5.0 over 96 h. ► Simple recovery/purification procedure for the rKmINU by ultrafiltration.

Keywords
Inulinase; Secretory expression; Inulin; Pichia pastoris; Enzyme activity
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High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 151–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us