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Stabilization of enzymes by the recombinant 30Kc19 protein

Paper ID Volume ID Publish Year Pages File Format Full-Text
34694 45039 2012 6 PDF Available
Title
Stabilization of enzymes by the recombinant 30Kc19 protein
Abstract

In previous studies, we reported that the 30K protein originating from the silkworm inhibited apoptosis in mammalian cells. In this work, we demonstrated that the 30Kc19 protein, which is most abundant 30K protein in silkworm hemolymph, also enhanced enzyme stability. When the recombinant 30Kc19 protein was supplemented into distilled–deionized water containing alkaline phosphatase or horseradish peroxidase, deactivation of both enzymes induced by non-buffered DDW was significantly suppressed. The increase in enzyme stability due to the presence of 30Kc19 was similar to that observed for bovine serum albumin, which is commonly used in conventional enzyme reactions. The decrease in enzyme activity due to long-term storage in different buffer systems was also inhibited by 30Kc19. The 30Kc19 protein structure was shown to play a vital role in stabilizing the enzyme. These results imply that the recombinant 30Kc19 protein hold promise for use as an additive to increase or maintain enzyme activity.

► The recombinant 30Kc19 protein enhanced enzyme stability. ► This may be a very practical way to stabilize or increase most of conventional enzymes. ► Our paper reports for the first time the insect-origin additive to preserve enzyme stability. ► Our findings dramatically increase the stability of alkaline phosphatase and horse raddish peroxidase. ► This will therefore be of very high interest to a broad international scientific and engineering audience.

Keywords
Enzyme stability; 30Kc19; Bovine serum albumin; Alkaline phosphatase; Horseradish peroxidase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 1, January 2012, Pages 164–169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us