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Mathematical modelling approach for concentration and productivity enhancement of 1,3-propanediol using Clostridium diolis

Paper ID Volume ID Publish Year Pages File Format Full-Text
3470 172 2012 8 PDF Available
Title
Mathematical modelling approach for concentration and productivity enhancement of 1,3-propanediol using Clostridium diolis
Abstract

1,3-Propanediol (1,3-PD) is an organic compound of immense importance particularly for polycondensation reactions to synthesise polyesters, polyethers and polyurethanes. Batch cultivation of obligate anaerobe Clostridum diolis DSM15410 for 1,3-PD production using statistically optimised medium was attempted in the present investigation. A mathematical model for the description of batch 1,3-PD production kinetics was then proposed which also incorporated substrate/product inhibition data. The kinetic parameters of the model were identified by non-linear regression technique using batch experimental data. The developed model adequately described the experimental data to the extent of 99% accuracy as tested by statistical validity test. The batch model was then extrapolated to fed-batch cultivation primarily to identify fresh nutrient feeding strategies (off-line on the computer) for enhanced production of 1,3-PD. Experimental implementation of one such fed-batch fermentation strategy involving constant feed rate and subsequent comparison of the observed kinetics with model simulation further established the accuracy of the developed model. A 1,3-PD concentration of 61.2 g/L with a productivity of 1.02 g/L/h of 1,3-PD was obtained in this mathematical-model guided fed-batch fermentation which is the highest 1,3-PD concentration ever reported in literature using this strain.

► Application of mathematical modelling for optimization of 1,3-PD production has been attempted. ► Batch mathematical model for 1,3-PD production has been proposed. ► Model parameters identified by non-linear regression technique using original batch experimental data. ► Proposed model extrapolated to fed-batch for enhanced 1,3-PD production. ► Highest 1,3-PD concentration ever reported in literature achieved using model-based fed-batch cultivation.

Keywords
1,3-Propanediol; Clostridium diolis; Modelling; Glycerol; Fed-batch culture; Kinetic parameters
First Page Preview
Mathematical modelling approach for concentration and productivity enhancement of 1,3-propanediol using Clostridium diolis
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 68, 15 October 2012, Pages 34–41
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering