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Potential use of nylon scouring pad cubes attachment method for pectinase production by Aspergillus niger HFD5A-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
34713 45040 2014 8 PDF Available
Title
Potential use of nylon scouring pad cubes attachment method for pectinase production by Aspergillus niger HFD5A-1
Abstract

•Cell immobilization improved fungal growth and pectinase production significantly.•Immobilization increased 335.0% of pectinase production compared to free cells.•Scouring pad cubes are good support matrix to immobilize the fungal cells.•Culture condition can impact fungal growth and pectinase production in immobilization.

This study investigated the novel use of scouring pad cubes as a support matrix for immobilization of fungal cell to enhance the pectinase production. Nylon scouring pad cubes were used for immobilized Aspergillus niger HFD5A-1 cells for pectinase production in flask submerge fermentation system. The enzyme activity of immobilized cell in scouring pad cubes gave higher activity compared to free cells. Various physical parameters for culture condition were studied to evaluate its effects on pectinase production. The maximum enzyme activity obtained was 11.05 U/mL on the 6th day of cultivation after using the optimized parameters of 6 scouring pad cubes, 1 × 107 spores/mL of inoculum size, agitation speed of 150 rpm and incubated at 30 °C. The use of nylon scouring pad cubes gave an increment of about 335.0% of pectinase production (11.05 U/mL) compared to free cells (2.54 U/mL). The results therefore show scouring pad cubes could be a favorable carrier to immobilize the fungal cells for higher enzyme production in submerged fermentation.

Keywords
Aspergillus niger; Immobilization; Nylon scouring pad; Pectinase; Optimization; Submerged fermentation
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Potential use of nylon scouring pad cubes attachment method for pectinase production by Aspergillus niger HFD5A-1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 4, April 2014, Pages 660–667
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us