fulltext.study @t Gmail

His-tagged Horse Liver Alcohol Dehydrogenase: Immobilization and application in the bio-based enantioselective synthesis of (S)-arylpropanols

Paper ID Volume ID Publish Year Pages File Format Full-Text
34730 45042 2013 9 PDF Available
Title
His-tagged Horse Liver Alcohol Dehydrogenase: Immobilization and application in the bio-based enantioselective synthesis of (S)-arylpropanols
Abstract

•Immobilization of His-tagged HLADH was successfully reported.•Effect of pH, temperature and organic co-solvents was investigated.•Application of the new imm-His-HLADH on the reduction of aldehydes was investigated.•Chemoenzymatic synthesis of enantiopure Profenols via DKR with His-HLADH was reported.

The novel histidine-tagged Horse Liver Alcohol Dehydrogenase (His-HLADH-EE) was successfully purified and covalently immobilized onto a solid support in a one-step procedure through a metal-directed technique. A full characterization of the immobilized enzyme was carried out. Effects of pH, temperature and organic co-solvents were deeply investigated and they showed a shift in the optimum pH with respect to the free form as well as increased stability to temperature and solvents. The immobilized His-HLADH-EE proved to be effective as catalyst in the reduction of aliphatic and aromatic aldehydes. Application of the free and immobilized His-HLADH-EE to the chemo-enzymatic synthesis of (S)-Profenols demonstrated enhanced enantioselectivity and high reusability of the immobilized form. The achievement of a robust and effective immobilization of an alcohol dehydrogenase substantiated the use of biocatalytic reduction in the synthesis of primary alcohols and valuable chiral intermediates especially for pharmaceutical industries.

Keywords
Alcohol dehydrogenases; Immobilization; Enantioselective reduction; Profenols; Dynamic kinetic resolution; Horse Liver Alcohol Dehydrogenase
First Page Preview
His-tagged Horse Liver Alcohol Dehydrogenase: Immobilization and application in the bio-based enantioselective synthesis of (S)-arylpropanols
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issues 5–6, May–June 2013, Pages 810–818
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering