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Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
34732 45042 2013 7 PDF Available
Title
Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction
Abstract

•Physisorption of Mucor Miehei Lipase onto ordered mesoporous silica.•High wall thickness is required to protect the support upon enzyme adsorption.•Mucor Miehei Lipase adsorption follows Langmuir isotherm.•Biocatalyst used for the methanolysis of colza in the absence of nonpolar solvents.•Good transesterification yields obtained by the addition of small amounts of water.

Herein, we report the design of a biocatalyst by the immobilization of Mucor Miehei Lipase (Mm-L) onto mesoporous silica materials. Supports with different pore diameters have been considered. Infrared spectroscopy was used to determine the adsorption isotherms in different pH conditions.Then, the biocatalyst was tested for the methanolysis of colza oil. The production of methyl esters was monitored over time by gas chromatography coupled to a mass spectrometer. The results show that to reach maximum performance of the biocatalyst, a certain amount of water is required (5 wt.%). By using ratio lower than the stoichiometry (1:1), the methanol conversion is completed and high transesterification yields could be obtained even in the absence of non polar solvents (i.e. hexane). Herein, the lipase uses the fatty substrate as lipophilic interface required for the opening of the active site of the enzyme.

Keywords
Lipase; Ordered mesoporous silica; Immobilization; Biocatalyst; Methanolysis; Colza oil
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issues 5–6, May–June 2013, Pages 831–837
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
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