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Recovery and characterization of proteinacious material recovered from thermal and alkaline hydrolyzed specified risk materials

Paper ID Volume ID Publish Year Pages File Format Full-Text
34739 45042 2013 8 PDF Available
Title
Recovery and characterization of proteinacious material recovered from thermal and alkaline hydrolyzed specified risk materials
Abstract

•We characterized hydrolysates of cattle tissues known as specified risk materials.•We measured protein cleavage as function of hydrolysis protocol.•We identified the reactive functionalities available.•We suggested possible industrial applications.

The purpose of this study was to characterize proteinacious material recovered from cattle tissues known as specified risk materials (SRM) hydrolyzed according to two protocols approved by the Canadian Food Inspection Agency and Food and Drug Administration and to evaluate its potential for non-food/feed value-added applications. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), size exclusion high performance liquid chromatography (SEC-HPLC), and free amino acid determination methods were adopted to measure the extent of protein cleavage and the average molecular weight of hydrolyzates, and to identify the reactive functionalities available. Molecular weight analysis revealed that the alkaline hydrolysis protocol cleaved the proteins more severely than thermal hydrolysis. Both protocols narrowed the molecular weight distribution of the proteins and increased their solubility in water. Several active functional groups amenable to further chemical processing were identified in the protein hydrolyzates. In conclusion, this work demonstrated that both hydrolytic protocols can be adopted as pre-processing steps to produce safe and renewable proteinacious feedstock for industrial applications.

Keywords
Specified risk material; Thermal hydrolysis; Alkaline hydrolysis; Prions; Bovine spongiform encephalopathy
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Recovery and characterization of proteinacious material recovered from thermal and alkaline hydrolyzed specified risk materials
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issues 5–6, May–June 2013, Pages 885–892
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us