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Induction of apoptosis by collinin from Zanthoxylum schinifolium is mediated via mitochondrial pathway in human Jurkat T cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
34748 45042 2013 10 PDF Available
Title
Induction of apoptosis by collinin from Zanthoxylum schinifolium is mediated via mitochondrial pathway in human Jurkat T cells
Abstract

•An apoptogenic ingredient collinin was isolated from the leaves of Zanthoxylum schinifolium.•Multidomain pro-apoptotic Bcl-2 proteins Bak and Bax were activated by collinin.•Δψm loss and caspase cascade activation, leading to PARP degradation, was induced by collinin.•Collin-induced apoptotic events were abrogated by overexpression of Bcl-2.•Either FADD-deficiency or caspase-8-deficiency failed to affect the sensitivity of Jurkat T cells to collinin-induced apoptosis.

Collinin, which was isolated from the leaves of Zanthoxylum schinifolium, could exert cytotoxic effect on various human tumor cells with IC50 values in the range of 38.1–111.6 μM, whereas the IC50 value for human normal mammary epithelial MCF-10A cells was 124.4 μM. To examine the contribution of apoptosis to the cytotoxicity of collinin toward tumor cells, collinin-induced apoptotic events of Jurkat T cells transfected with vector (JT/Neo) were compared with those of Jurkat T cells transfected with Bcl-2 gene (JT/Bcl-2). Treatment of JT/Neo cells with collinin (30–60 μM) resulted in induction of sub-G1 peak representing apoptotic cells along with activation of Bak and Bax, mitochondrial membrane potential (Δψm) loss, activation of caspase-9, -3, -8, and -7, degradation of PARP, and DNA fragmentation dose-dependently, but these apoptotic events were abrogated by overexpression of Bcl-2, which could prevent the induced activation of Bak and Bax, and subsequent mitochondrial damage. Under these conditions, necrosis was not accompanied. Pretreatment of JT/Neo cells with the pan-caspase inhibitor z-VAD-fmk completely blocked collinin-induced apoptotic sub-G1 cells and caspase cascade activation, whereas it failed to suppress Bak activation and Δψm loss. Neither FADD-deficiency nor caspase-8-deficiency affected the susceptibility of Jurkat T cells to collinin-induced cytotoxicity and apoptotic cell death. These results demonstrate that the apoptogenic activity of collinin was mediated by the intrinsic mitochondrial apoptotic pathway which was preceded by activation of pro-apoptotic multidomain Bcl-2 family members Bak and Bax, mitochondrial damage, and resultant activation of caspase cascade, leading to PARP degradation, which could be regulated by Bcl-2.

Keywords
Zanthoxylum schinifolium; Collinin; Bak; Bax; Δψm loss; Caspase cascade
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Induction of apoptosis by collinin from Zanthoxylum schinifolium is mediated via mitochondrial pathway in human Jurkat T cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issues 5–6, May–June 2013, Pages 945–954
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us