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Preparation, isolation and identification of iron-chelating peptides derived from Alaska pollock skin

Paper ID Volume ID Publish Year Pages File Format Full-Text
34753 45042 2013 6 PDF Available
Title
Preparation, isolation and identification of iron-chelating peptides derived from Alaska pollock skin
Abstract

•Alaska pollock skin could be utilized to generate iron-chelating peptides.•Low molecular weight (below 500 Da) hydrolysate derived from Alaska pollock skin exhibited high chelating activity.•Ser-Cys-His (MW: 345 Da) was identified as an iron chelating peptide.•Chelating activity of Ser-Cys-His should attribute to both its low molecular mass and amino acid composition.

Alaska pollock skin as aquatic by-product was usually discarded in food processing. Iron deficiency has been a nutritional problem for a long period. In this study, Alaska pollock skin was used to generate iron-chelating peptides after being treated by commercial enzymes. Sequential chromatography, including immobilized metal affinity chromatography (IMAC) and reversed phase high performance liquid chromatography (RP-HPLC), was used for capturing and purifying iron-chelating peptides. One tripeptide, with high iron-chelating activity, was obtained and identified by electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS). The amino acid sequence of the iron-chelating peptide was identified to be Ser-Cys-His (MW: 345 Da). These results show that Alaska pollock skin could be utilized to generate iron-chelating peptides as an iron supplement in functional food industry.

Keywords
Iron-chelating peptides; Bioavailability; Iron; Ser-Cys-His; Immobilized metal affinity chromatography (IMAC); Tandem mass spectrum (MS/MS)
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Preparation, isolation and identification of iron-chelating peptides derived from Alaska pollock skin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issues 5–6, May–June 2013, Pages 988–993
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us