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Production of yeast chitin–glucan complex from biodiesel industry byproduct ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
34776 45047 2012 6 PDF Available
Title
Production of yeast chitin–glucan complex from biodiesel industry byproduct ☆
Abstract

Crude glycerol from the biodiesel industry was used as carbon source for high cell density fed-batch cultivation of Pichia pastoris aiming at producing a chitin–glucan complex (CGC). More than 100 g L−1 biomass was obtained in less than 48 h. The yield of biomass on a glycerol basis was 0.55 g g−1 during the batch phase and 0.63 g g−1 during the fed-batch phase. The chitin–glucan complex was recovered from the yeast cell wall by hot alkaline extraction. CGC content in the cell wall was found to be relatively constant throughout the cultivation (18–26%) with a volumetric productivity of 1.28 g L−1 h−1 at the end of the fed-batch phase. The molar ratio of chitin:β-glucan in the extracted biopolymer was 16:84, close to other CGC extracted from Aspergillus biomass. The extracted polymer was characterized by Differential Scanning Calorimetry (DCS) and solid-state Nuclear Magnetic Resonance (NMR) spectroscopy and compared with commercial biopolymers, namely, crab shell chitin and/or chitosan, algal β-glucan (laminarin) and fungal chitin–glucan complex (kiOsmetine).

Keywords
Chitin–glucan complex; Pichia pastoris; Glycerol
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Production of yeast chitin–glucan complex from biodiesel industry byproduct ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 11, November 2012, Pages 1670–1675
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us