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BUL: A novel lectin from Bauhinia ungulata L. seeds with fungistatic and antiproliferative activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
34783 45048 2014 7 PDF Available
Title
BUL: A novel lectin from Bauhinia ungulata L. seeds with fungistatic and antiproliferative activities
Abstract

•A new lectin from Bauhinia ungulata seeds (BUL) was purified and characterized.•BUL showed antifungal activity against phytopathogenic species.•BUL exhibits antiproliferative effect against cells of human colon adenocarcinoma.

A new galactose-binding lectin, termed BUL, has been purified from seeds of Bauhinia ungulata L. (Caesalpinoideae) by precipitation with solid ammonium sulfate followed by agarose–lactose affinity chromatography. B. ungulata lectin strongly agglutinated rabbit erythrocytes, both native and treated with proteolytic enzymes, and was inhibited by d-galactose and d-galactose-derived sugars, especially N-acetyl-d-galactosamine. BUL was shown to be a stable glycoprotein, maintaining its hemagglutinating activity after incubation at wide ranges of temperature and pH, but not after incubation with EDTA. By SDS-PAGE analysis, purified BUL showed an electrophoretic profile consisting of a single band with apparent molecular mass of 30 kDa. BUL showed intrinsic fluorescence typical of folded globular proteins, and CD spectra of lectin in the native state showed a predominance of β-sheet secondary structure. The N-terminal amino acid sequence of 19 residues showed a high sequential similarity to other galactose-specific lectins from the Bauhinia genus. In addition, BUL showed antifungal activity against phytopathogenic species and showed in vitro antiproliferative activity against the HT-29 cell line of human colon adenocarcinoma in a dose-dependent manner.

Keywords
Lectin; Bauhinia ungulata; Fungistatic and antiproliferative activities
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BUL: A novel lectin from Bauhinia ungulata L. seeds with fungistatic and antiproliferative activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 2, February 2014, Pages 203–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us