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Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
34817 45051 2014 9 PDF Available
Title
Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production
Abstract

•New method for microbial cell immobilization.•Highly macroporous structure consisting of crosslinked aggregated bacterial cells.•High production of butanol in batch using immobilized Clostridium acetobutylicum.

In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3–5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production.

Keywords
ABE, acetone-butanol-ethanol; OxDex, oxidized dextran; GTA, glutaraldehyde; PEI, polyethyleneimine; PVA, poly(vinyl alcohol)Cell immobilization; Cryogel; Butanol; Clostridium acetobutylicum
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Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 1, January 2014, Pages 10–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us