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Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies

Paper ID Volume ID Publish Year Pages File Format Full-Text
34818 45051 2014 6 PDF Available
Title
Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies
Abstract

•Three target single bacterial colonies were isolated from human intestinal bacteria.•More than 90% SDG in flaxseed were converted to END by the co-culture of three strains.•The method for producing END is meaningful for utilizing flaxseed resources.

Enterodiol (END) possesses the estrogenic and antiestrogenic activities, which could prevent the development of breast cancer and prostate cancer, as well as menopausal syndrome.Previous studies in our laboratory set up a bio-transformation method for largely yielding secoisolariciresinol (SECO) from the substrate of defatted flaxseeds by strain Bacteroides uniformis ZL-I. In this research, another two single colonies, designated as strain ZL-II and strain ZL-III, were isolated, which were closely related to Eubacterium limosum species (ZL-II), and Eggerthella lenta species (ZL-III) on the basis of 16S rRNA gene sequence data. Under the combining actions of strains ZL-(I + II + III), END could be produced from defatted flaxseed directly, ZL-II was proved to possess the activities of demethylation, while ZL-III had the activities of dehydroxylation. Secoisolariciresinol diglucoside (SDG) existed in the form of oligomeric with 3-hydroxy-3-methyl glutaric acid in flaxseed could be efficiently transformed into END under the co-culture of strains ZL-(I + II + III), with the conversion rate of more than 90%. The method for mass-producing END from defatted flaxseed reported here is meaningful not only for the medicinal values of END but also for the resource utilization of flaxseed materials.

Keywords
Enterodiol; Defatted flaxseed; Biotransformation; Bacteroides uniformis; Eubacterium limosum; Eggerthella lenta
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Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 1, January 2014, Pages 19–24
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us