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Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
34819 45051 2014 8 PDF Available
Title
Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli
Abstract

•Single microbial conversion of d-xylose to 1,2,4-butanetriol is first reported.•Pathway thermodynamics, product toxicity and redox in E. coli were investigated.•Expression of two genes from two different bacteria completes the four-step pathway.•Highest titer achieved upon deletion of native d-xylose and d-xylonate metabolism.

The compound 1,2,4-butanetriol (BT) is a valuable chemical used in the production of plasticizers, polymers, cationic lipids and other medical applications, and is conventionally produced via hydrogenation of malate. In this report, BT is biosynthesized by an engineered Escherichia coli from d-xylose. The pathway: d-xylose → d-xylonate → 2-keto-3-deoxy-d-xylonate → 3,4-dihydroxybutanal → BT, was constructed in E. coli by recruiting a xylose dehydrogenase and a keto acid decarboxylase from Caulobacter crescentus and Pseudomonas putida, respectively. Authentic BT was detected from cultures of the engineered strain. Further improvement on the strain was performed by blocking the native d-xylose and d-xylonate metabolic pathways which involves disruption of xylAB, yjhH and yagE genes in the host chromosome. The final construct produced 0.88 g L−1 BT from 10 g L−1d-xylose with a molar yield of 12.82%. By far, this is the first report on the direct production of BT from d-xylose by a single microbial host. This may serve as a starting point for further metabolic engineering works to increase the titer of BT toward industrial scale viability.

Keywords
d-Xylose; 1,2,4-Butanetriol; Escherichia coli; Metabolic engineering
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Direct bioconversion of d-xylose to 1,2,4-butanetriol in an engineered Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 1, January 2014, Pages 25–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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Any Questions? feel free to contact us