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Biochemical characterization of an endoxylanase from Pseudozyma brasiliensis sp. nov. strain GHG001 isolated from the intestinal tract of Chrysomelidae larvae associated to sugarcane roots

Paper ID Volume ID Publish Year Pages File Format Full-Text
34826 45051 2014 7 PDF Available
Title
Biochemical characterization of an endoxylanase from Pseudozyma brasiliensis sp. nov. strain GHG001 isolated from the intestinal tract of Chrysomelidae larvae associated to sugarcane roots
Abstract

•Pseudozyma brasiliensis sp. nov. strain was isolated from the Chrysomelidae larvae.•This basidiomycetous yeast produces a cellulase-free xylanase designated PbXynA.•PbXynA has sigmoidal kinetic behavior with elevated speed conversion substrate–products.•PbXynA is highly activated by bivalent cations such as Mg2+, Zn2+ and Ca2+.•PnXynA has a high specific activity and produces xylooligosaccharides.

Endo-xylanases play a key role in the hydrolysis of xylan and recently they have attracted much attention due to their potential applications on the biofuel and paper industries. We isolated a Pseudozyma brasiliensis sp. nov. strain from the intestinal tract of Chrysomelidae larvae that parasitize sugarcane roots. This basidiomycetous yeast produces a xylanase designated PbXynA which was purified and characterized. The molecular weight of PbXynA is 24 kDa, it belongs to the GH11 family and its optimum pH and optimum temperature are 4.0 and 55 °C, respectively. PbXynA has as secondary structure predominantly β-sheets and sigmoidal kinetic behavior with elevated speed conversion from substrate-to-products (Vmax = 2792.0 μmol product/min/mg protein). It is highly activated by bivalent cations such as Ca2+, however in the presence of Cu2+ xylanase activity was inhibited. It has a high specific activity and produces xylooligosaccharides that have a variety of industrial applications, indicating PbXynA has a great biotechnological potential.

Keywords
Pseudozyma brasiliensis; Endoxylanase; Biochemical characterization; GH11
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Biochemical characterization of an endoxylanase from Pseudozyma brasiliensis sp. nov. strain GHG001 isolated from the intestinal tract of Chrysomelidae larvae associated to sugarcane roots
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 49, Issue 1, January 2014, Pages 77–83
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us