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Cellobiohydrolase secretion by yeast: Current state and prospects for improvement

Paper ID Volume ID Publish Year Pages File Format Full-Text
34841 45052 2013 12 PDF Available
Title
Cellobiohydrolase secretion by yeast: Current state and prospects for improvement
Abstract

Lignocellulose is an abundant and renewable feedstock for the production of such commodities as fuels and chemicals, provided that a low-cost technology can be developed to overcome its recalcitrance. Organisms that hydrolyze the sugar polymers in lignocellulose to produce a valuable product at a high rate would significantly reduce the costs of current conversion technologies. To develop yeasts, such as Saccharomyces cerevisiae, for such consolidated bioprocessing (CBP), a secreted heterologous cellulolytic enzyme system must be engineered into it. While considerable progress has been made in this regard, the secretion of cellobiohydrolases (CBHs) at levels required for crystalline cellulose hydrolysis has remained elusive until recently. Recent results suggest the existence of a compatibility factor for the expression of foreign genes in a host and that expression of some genes or their products exerted varying degrees of stress on the cell. The secretion machinery of yeasts is a multi-step process and each step is directed and regulated by several proteins, providing a vast array of targets that can be manipulated to enhance heterologous protein secretion. This review assesses the current state of the field with respect to CBH secretion in yeast and the options for enhancing yeast secretion capacity through strain engineering.

► Cellobiohydrolases are key enzymes in cellulose hydrolysis and biomass conversion. ► Expression of cellobiohydrolases in yeasts have met with varying degrees of success. ► Cellobiohydrolases produced in yeast were often aberrant in folding or glycosylation. ► Cellobiohydrolase expression in yeast caused gene dependent cellular stress. ► The yeast secretion pathway can be engineered for cellulase secretion enhancement.

Keywords
Consolidated bioprocessing; Cellulase secretion; Cellobiohydrolase; Heterologous protein secretion
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Cellobiohydrolase secretion by yeast: Current state and prospects for improvement
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 1, January 2013, Pages 1–12
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us