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Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse pancreatic β-cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
34847 45052 2013 10 PDF Available
Title
Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse pancreatic β-cells
Abstract

Cell behavior is determined by intrinsic characteristics and complex interactions with microenvironments. This study demonstrated the performance of a murine pancreatic β-cell line, MIN-6, cultured on tissue-culture polystyrene (TCPS), gelatin, type I collagen, and type IV collagen dishes. MIN-6 cells aggregated as clusters on gelatin, type I collagen, and type IV collagen, which was different from the epithelial morphology of cells grown on TCPS. The diameter and survival rate of aggregated cells did not differ significantly regardless of whether the cells were grown on gelatin or type I collagen, while smaller clusters were observed on type IV collagen. Compared with the monolayers on TCPS, the clusters had a higher insulin stimulation index. The mRNA expression levels of Ins1, Pdx-1, NeuroD1 and connexin 36 were upregulated in clusters relative to monolayers. Conversely, E-cadherin and MafA were downregulated when cells were grown on type IV collagen. Monolayers or cell aggregates grown on type IV collagen were subsequently transplanted into diabetic C57BL/6 mice. Animals that received both monolayers and clusters had decreased blood glucose levels and regained body weight. However, the area under curve for the intraperitoneal glucose tolerance test showed that clusters exhibited superior in vivo performance. This study reveals that a type IV collagen substrate promotes β-cell clustering, regulates gene expression and enhances in vivo performance.

► Pancreatic β-cell formed clusters on gelatin, type I collagen and type IV collagen different from the epithelial morphology of those grown on tissue-culture polystyrene. ► PCR revealed that cell aggregation regulates insulin gene expression to promote insulin secretion. ► Diabetic mice received both monolayers and clusters had a decrease in blood glucose levels and an increase in body weights. ► Animal study showed clusters had superior in vivo performance.

Keywords
Pancreatic β-cell; Gelatin; Type I collagen; Type IV collagen; Cell cluster; Connexin 36
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Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse pancreatic β-cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 1, January 2013, Pages 58–67
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us