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Iron containing keratinolytic metallo-protease produced by Chryseobacterium gleum

Paper ID Volume ID Publish Year Pages File Format Full-Text
34858 45052 2013 8 PDF Available
Title
Iron containing keratinolytic metallo-protease produced by Chryseobacterium gleum
Abstract

Chryseobacterium gleum exhibited complete dissolution of whole chicken-feathers (10 g l−1, pH 8) after 72 h at 30 °C through synthesis of keratinolytic protease when inoculated at 1% (v/v). This enzyme was purified to 67-fold with yield of 2.25% having a specific activity of 1670 U mg−1 and ∼36 kDa Mw. MALDI-TOF MS of this keratinase showed some similarity with the keratinase peptides of Bacillus subtilis (BOFXJ2). The keratinase action was inhibited by EDTA, iodoacetamide and metal ions like mercury, copper and zinc (1 mM each), while it was enhanced by iron and calcium. Keratinase showed presence of 3 mM of Fe M−1 as tested by atomic absorption spectroscopy and addition of Fe in its apoenzyme retained about 79% of original residual feather degradation activity which portrayed it to be metalloprotease. Purified keratinase revealed significant degradation (85%) of feather concentrate (20 g l−1) to 3.9 μM ml−1 of free amino groups in 24 h at an initial pH of 8.0, 30 °C and 120 rpm shaking. This keratinase activity can be controlled precisely by presence of chemical or metal ions which could be of use in biotechnology industry while the culture can be used in poultry waste management.

► Chryseobacterium gleum was evaluated for feather keratin degradation. ► Keratinolytic protease of C. gleum was purified having relative Mw ∼36 kDa. ► Iron found in keratinolytic protease which enhanced its activity. ► Inhibition by EDTA confirmed its class as metallo-protease.

Keywords
Chryseobacterium gleum; Feather degradation; Keratinase; Iron; Metalloprotease; Poultry waste management
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Iron containing keratinolytic metallo-protease produced by Chryseobacterium gleum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 1, January 2013, Pages 144–151
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us