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A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ

Paper ID Volume ID Publish Year Pages File Format Full-Text
34861 45052 2013 7 PDF Available
Title
A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ
Abstract

A thermostable and organic solvent-tolerant lipase produced by Aneurinibacillus thermoaerophilus strain HZ was purified and characterised. The lipase was purified to apparent homogeneity with two steps: anion exchange chromatography on Q-Sepharose and gel filtration on Sephadex-G75. A final specific activity of 43.5 U/mg was obtained with an overall recovery of 19.7% and 15.6 purification fold. The molecular mass of the HZ lipase was estimated to be 50 kDa. The optimum pH for the activity of the purified HZ lipase was 7.0. The stability showed a broad range of pH values between pH 4.0 and 9.0 at 30 °C. The purified HZ lipase exhibited an optimum temperature of 65 °C with a half-life of 3 h and 10 min at 65 °C. The activity of the purified HZ lipase was stimulated in the presence of Ca2+. Organic solvents such as dimethyl sulfoxide (DMSO), methanol, n-tetradecane and n-hexadecane enhanced the lipase activity. Studies on the effect of oil showed that the lipase preferred natural oil, such as sunflower oil, over synthetic substrates.

► HZ lipase was the first report of thermostable and organic solvent-tolerant lipase from Aneurinibacillus genus. ► It was purified using two steps of purification to 15.6-fold purity and 19.7% recovery. ► The most influential parameters for optimum enzyme activity was determined. ► It had shown potential characteristic for industrial application.

Keywords
Aneurinibacillus thermoaerophilus strain HZ; Thermostable lipase; Organic solvent tolerant; Purification; Characterisation
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A new thermostable and organic solvent-tolerant lipase from Aneurinibacillus thermoaerophilus strain HZ
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 48, Issue 1, January 2013, Pages 169–175
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us