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Purification of angiotensin I-converting enzyme inhibitory peptides from a cowpea (Vigna unguiculata) enzymatic hydrolysate

Paper ID Volume ID Publish Year Pages File Format Full-Text
34871 45056 2011 9 PDF Available
Title
Purification of angiotensin I-converting enzyme inhibitory peptides from a cowpea (Vigna unguiculata) enzymatic hydrolysate
Abstract

Angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated and characterized from cowpea (Vigna unguiculata L. walp). Cowpea protein isolate was prepared by wet fractionation, extensively hydrolyzed with Flavourzyme® and filtered through four molecular weight cut-off (MWCO) membranes in a high performance ultrafiltration (UF) cell. The ultrafiltered fraction with the highest ACE inhibitory activity was purified using gel filtration chromatography followed by reverse-phase high performance liquid chromatography (RP-HPLC). The cowpea peptide inhibition (IC50) values were similar to those reported for many other natural ACE inhibitory peptides. The <1 kDa ultrafiltered fraction exhibited the highest biological activity. The observed amino acid composition suggests a substantial contribution of hydrophobic residues to the peptides’ inhibitory potency, which potentially acts via blocking of angiotensin II production. When hydrolyzed with the protease Flavourzyme®, cowpea V. unguiculata protein is a good source of ACE inhibitory peptides with potential applications in physiologically functional foods with antihypertensive activity.

Keywords
Vigna unguiculata L. walp; Hydrolysis; Purification; ACE inhibitors; Amino acid composition
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Purification of angiotensin I-converting enzyme inhibitory peptides from a cowpea (Vigna unguiculata) enzymatic hydrolysate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 4, April 2011, Pages 864–872
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us