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Activity, conformation and thermal stability of laccase and glucose oxidase in poly(ethyleneimine) microcapsules for immobilization in paper

Paper ID Volume ID Publish Year Pages File Format Full-Text
34888 45056 2011 8 PDF Available
Title
Activity, conformation and thermal stability of laccase and glucose oxidase in poly(ethyleneimine) microcapsules for immobilization in paper
Abstract

In this paper we report on the microencapsulation of glucose oxidase from Aspergillus niger (GOx) and laccase from Trametes versicolor (TvL) with the goal of immobilizing these enzymes in paper substrates to develop biosensors and bioreactors. Despite having high encapsulation efficiency, the technique caused a severe decrease (up to 65%) in the specific activities of both enzymes once microencapsulated. Encapsulated TvL presented lower KM and Vmax values compared to free laccase in solution, but the KM for GOx did not change after microencapsulation. TvL was found to interact strongly with poly(ethyleneimine) (PEI) in a manner similar to that expected from an uncompetitive inhibition mechanism, and fluorescence and circular dichroism spectroscopy demonstrated that significant alteration of the enzyme's conformation occurs in the presence of PEI, an effect of the cationic polymer that was not observed with GOx. Microencapsulation improved the thermal stability of GOx at temperatures up to 60 °C due to stabilization of its active conformation but reduced the thermal stability of laccase because of the increased coordination between PEI and copper atoms in the enzyme's active site. Preliminary GOx bioactive paper was fabricated, which could be potentially used as food packaging paper.

Keywords
Bioactive paper; Enzyme; Conformation; Microencapsulation; Stability; Immobilization
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Activity, conformation and thermal stability of laccase and glucose oxidase in poly(ethyleneimine) microcapsules for immobilization in paper
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 4, April 2011, Pages 993–1000
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us