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Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction

Paper ID Volume ID Publish Year Pages File Format Full-Text
34890 45056 2011 9 PDF Available
Title
Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction
Abstract

Carbonic anhydrase (CA) has been immobilized on chitosan beads and has been tested for its activity using p-NPA. CA immobilized chitosan beads were further tested for targeted application of carbonation reaction to convert CO2 to CaCO3. It was observed that onset time for precipitation of CaCO3 was 40 s for immobilized catalyst as compared to non-catalyzed system (100 s). Mechanistic and kinetic aspects have been addressed for immobilized catalyst as well as free CA using p-NPA assay. Km and Vmax of the immobilized CA was observed to be 2.36 mM and 0.54 μmoles/min/ml as compared to Km and Vmax of 0.87 mM and 0.93 μmoles/min, respectively, for free CA. Storage stability test was conducted up to 20 days and it was observed that at −20 °C, the Half Life Period (HLP) of immobilized CA was 216 h as compared to 192 h for free CA whereas at 25 °C the HLP for immobilized and free CA was observed to be 456 h and 408 h, respectively.

Keywords
Chitosan beads; Carbonic anhydrase; Immobilization; Carbonation reaction; Kinetics; Stability
First Page Preview
Immobilization of carbonic anhydrase on chitosan beads for enhanced carbonation reaction
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 4, April 2011, Pages 1010–1018
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering