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Performance improvement of araujiain, a cystein phytoprotease, by immobilization within calcium alginate beads

Paper ID Volume ID Publish Year Pages File Format Full-Text
34893 45056 2011 6 PDF Available
Title
Performance improvement of araujiain, a cystein phytoprotease, by immobilization within calcium alginate beads
Abstract

The effect of immobilization within alginate beads on the performance of araujiain in aqueous and non-aqueous media was studied. Optimum pH and temperature, thermal and pH-stability, kinetic parameters and operational stability were determined. Entrapped enzyme showed an increase in thermal and pH-stability, a high activity over the studied pH range (5.0–9.5), and a shift in optimum temperature from 37 °C to 70 °C. Immobilized araujiain retained 95% of their initial activity after 45 days of storage at 4 °C, and it maintained 78% of activity after 20 hydrolytic reaction cycles. Furthermore, it showed a good performance in non-aqueous medium, used for the peptide synthesis of interest for the food industry, with a yield 2.6-fold greater than using free enzyme. The entrapped enzyme was more stable over a wide range of temperatures, pH, and storage time as compared to free enzyme.

Keywords
Araujiain; Cystein phytoprotease; Enzymatic peptide synthesis; Immobilization; Alginate beads
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 4, April 2011, Pages 1029–1034
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us