fulltext.study @t Gmail

Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Paper ID Volume ID Publish Year Pages File Format Full-Text
3490 172 2012 8 PDF Available
Title
Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability
Abstract

A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5 nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► The pore surface of SBA-15 was coated with an extremely thin layer of carbon. ► The stability of SBA-15 toward high pH has successfully increased by coating it with carbon. ► The Glu/carbon/SBA-15 catalyzed the reaction for production of l-theanine under high pH conditions.

Keywords
Carbon; Mesoporous silica; Enzyme immobilization
First Page Preview
Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 68, 15 October 2012, Pages 207–214
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering