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Optimizing amino acid composition of CHO cell culture media for a fusion protein production

Paper ID Volume ID Publish Year Pages File Format Full-Text
34901 45059 2011 7 PDF Available
Title
Optimizing amino acid composition of CHO cell culture media for a fusion protein production
Abstract

The amino acid composition of CHO cell culture media for manufacturing an antibody fusion protein (B1) was optimized using data obtained through metabolic flux analysis. The metabolic flux analysis was performed using the metabolic rates derived from two semi-steady states of continuous culture. The amino acid composition was the same for media used in both the fed-batch and continuous cultures, which made the modification of amino acid composition suggested by the metabolic flux analysis applicable to the fed-batch culture. The flux analysis indicated that methionine, tryptophan, asparagine and serine were limiting, while alanine, arginine, glutamine and glycine were in excess at the semi-steady states. Based on this result, the limiting amino acids were increased while those in excess were reduced to obtain balanced amino acid composition in both the batch and feed media. The fed-batch cell cultures using the modified media generated less lactate and ammonia, and more significantly, increased peak cell densities by 55% and B1 protein titer by 27% without any impact on final sialic acid content, a quality attribute of the B1 protein.

Keywords
Medium optimization; Productivity improvement; Metabolic flux analysis; Nutrient demands; Toxic metabolites
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 7, July 2011, Pages 1423–1429
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us