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Oleaginous yeast Yarrowia lipolytica mutants with a disrupted fatty acyl-CoA synthetase gene accumulate saturated fatty acid

Paper ID Volume ID Publish Year Pages File Format Full-Text
34903 45059 2011 6 PDF Available
Title
Oleaginous yeast Yarrowia lipolytica mutants with a disrupted fatty acyl-CoA synthetase gene accumulate saturated fatty acid
Abstract

Fatty acyl-CoA synthetases are critical enzymes involved in lipid metabolism. The oleaginous yeast Yarrowia lipolytica is currently generating interest in biofuel research due to its ability to convert raw materials into value-added end products. In this study, the putative acyl-CoA synthetase gene YAL1 in Y. lipolytica was interrupted with the copper resistance (CRF1) gene to allow selection without antibiotics to facilitate industrial applications. Deletion of YAL1 led to reduced acyl-CoA synthetase activity. Furthermore, the fatty acid profile and lipid content of the mutant were different from the wild-type strain. The ratio of saturated to unsaturated fatty acids increased 6-fold, and the total lipid production of the mutant strain increased to 1.47-fold of the wild-type strain. The results indicate that YAL1 in Y. lipolytica is involved in fatty acid elongation and desaturation, whereas the homologous, highly conserved FAA1 gene from Saccharomyces cerevisiae was shown to be responsible for fatty acid activation. The increased ratio of saturated to unsaturated fatty acids would result in a higher combustion value and better oxidative stability for biofuel products obtained from the fatty acids from the engineered Y. lipolytica mutant.

Keywords
Acyl-CoA synthetase; Fatty acid; Oleaginous yeast; Yarrowia lipolytica
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Oleaginous yeast Yarrowia lipolytica mutants with a disrupted fatty acyl-CoA synthetase gene accumulate saturated fatty acid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 7, July 2011, Pages 1436–1441
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us