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Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
34904 45059 2011 7 PDF Available
Title
Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene
Abstract

The INU1 gene encoding exo-inulinase cloned from Kluyveromyces marxianus CBS 6556 was ligated into the expression plasmid pINA1317 and expressed in the cells of the yeast Yarrowia lipolytica with high content of protein. The activity of the secreted inulinase was found to be 43.1 ± 0.9 U/ml after cell growth for 80 h. When the engineered yeast cells were grown in the medium containing 4.0% inulin in 2-l fermentor, crude protein in the cells and cell mass could reach 47.5% and 20.1 g/l, respectively within 72 h. Furthermore, when the engineered yeast cells were grown in the medium containing 8.0% the meal of Jerusalem artichoke tuber in 2-l fermentor, crude protein and cell mass could reach 53.7% and 20.8 g/l, respectively within 80 h. Therefore, the engineered Y. lipolytica could be further used to produce single cell protein from inulin and inulin-containing materials.

Keywords
Expression; Single cell protein; Inulinase; Yarrowia lipolytica; Marine yeasts
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Direct conversion of inulin into single cell protein by the engineered Yarrowia lipolytica carrying inulinase gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 7, July 2011, Pages 1442–1448
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us