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Purification, characterization and immobilization of urease from Momordica charantia seeds

Paper ID Volume ID Publish Year Pages File Format Full-Text
34911 45059 2011 6 PDF Available
Title
Purification, characterization and immobilization of urease from Momordica charantia seeds
Abstract

Ureases are used in beverage, fertilizer and pharmaceutical industries. We report purification, characterization and a simple method for immobilization of urease form Momordica charantia seeds. The purification of the enzyme is a two steps process involving anion exchange chromatography using DEAE–Sepharose matrix followed by size exclusion chromatography. The fold purification of the enzyme was found to be 6.5. The estimated molecular mass and Km of the enzyme were 174.5 kDa and 34 ± 0.5 mM, respectively. The purified urease was immobilized on chitosan beads activated with glutaraldehyde. The activation of chitosan beads and immobilization were confirmed by Fourier Transform Infrared (FTIR) spectrometry and Scanning Electron Microscope (SEM), respectively. Moreover, Energy Dispersive X-ray (EDX) analysis shows increased percentage of nitrogen which further confirms immobilization. The efficiency of immobilization was found to be approximately 97%. The immobilized enzyme retains 60% activity till fourteen successive batches of enzymatic reactions which makes it a highly stable product. Comprehensively, the enzyme shows desirable properties for various applications.

Keywords
Chitosan; Immobilization; Momordica charantia; Purification; Urease
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Purification, characterization and immobilization of urease from Momordica charantia seeds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 46, Issue 7, July 2011, Pages 1486–1491
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us