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Bioactive constituents and anti-proliferative properties of supercritical carbon dioxide Salvia miltiorrhiza extract in 3T3-L1 adipocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
34923 45060 2012 9 PDF Available
Title
Bioactive constituents and anti-proliferative properties of supercritical carbon dioxide Salvia miltiorrhiza extract in 3T3-L1 adipocytes
Abstract

The present study examined the anti-proliferative properties of aqueous (WESM), ethanolic (EESM) and supercritical carbon dioxide (SCESM-5) extracts of Salvia miltiorrhiza in 3T3-L1 adipocytes, and the contents of bioactive compounds salvianolic acid B (SAB), cryptotanshinone (CTS), tanshinone I (TS-I) and tanshinone IIA (TS-IIA). Results showed that WESM contained mainly SAB, whereas EESM possessed all the four bioactive compounds, but its concentrations of CTS, TS-I and TS-IIA were 2–3-fold lower than SCESM-5. SCESM-5 demonstrated the most potent inhibitory effect on 3T3-L1 cell proliferation, and induction of cell apoptosis and cell cycle arrest. Further studies showed that it enhanced intracellular ROS level, down-regulated CD95 (APO-1/CD95), Bcl-2 and PPAR-γ, up-regulated Bax, AMPK and p-AMPK, and caused activation of caspase-3 and PARP cleavage, as well as JNK and ERK activation and phosphorylation. Pretreatments with antioxidant (Vit E), caspase-3 (z-DEVD-fmk) and ERK (PD98059) inhibitors significantly suppressed the SCESM-5-induced ROS production and cell death. Caspase-3 inhibitor efficiently blocked the Bax expression, caspase-3 activation and PARP cleavage, while ERK inhibitor suppressed the ERK phosphorylation after SCESM-5 treatment. This study concludes that CTS, TS-I and TS-IIA of SCESM-5 may have contributed to the potent anti-proliferative activity through the MAPK and AMPK pathways in 3T3-L1 adipocytes.

► The first to use the supercritical carbon dioxide technique for preparing Salvia miltiorrhiza or Danshen extract (SCESM-5), and comparing its content of bioactive compounds salvianolic acid B (SAB), cryptotanshinone (CTS), tanshinone I (TS-I) and tanshinone IIA (TS-IIA) with extracts prepared by other conventional methods. ► SCESM-5 showed excellent anti-proliferative effects on 3T3-L1 adipocytes; it demonstrated a greater potency in inhibiting 3T3-L1 cell proliferation, and inducing cell apoptosis and cell cycle arrest than extracts obtained with conventional methods. ► First to note that CTS, TS-I and TS-IIA have contributed significantly to the anti-proliferative potency of SCESM-5 in 3T3-L1 adipocytes, and its mechanisms of action are mainly mediated by the MAPK and AMPK pathways, and the modification of the Bcl-2 family proteins and PPAR-γ expression.

Keywords
Salvia miltiorrhiza; Supercritical carbon dioxide; ROS; PPAR-γ; MAPK; AMPK
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Bioactive constituents and anti-proliferative properties of supercritical carbon dioxide Salvia miltiorrhiza extract in 3T3-L1 adipocytes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 216–224
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us