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Purification and partial characterisation of a thermostable xylanase from salt-tolerant Thermobifida halotolerans YIM 90462T

Paper ID Volume ID Publish Year Pages File Format Full-Text
34924 45060 2012 4 PDF Available
Title
Purification and partial characterisation of a thermostable xylanase from salt-tolerant Thermobifida halotolerans YIM 90462T
Abstract

ThxynA, an extracellular xylanase of T. halotolerans YIM 90462T, was purified to homogeneity from a fermentation broth by ultra-filtration, ammonium sulphate precipitation, hydrophobic chromatography and ion exchange chromatography. The purified xylanase has a molecular mass of 24 kDa and is optimally active at 80 °C and pH 6.0. The enzyme is stable over a broad pH range (pH 6.0–10.0) and shows good thermal stability when incubated at 70 °C for 1 h. The Km and Vmax values of the enzyme are 11.6 mg/mL and 434 μmol mg−1 min−1, respectively, using oat spelt xylan as a substrate. Moreover, the enzyme seemingly has both xylanase activity and cellulase activity. These unique properties suggest that it may be useful for industrial applications.

► The stain we used can grow in a high temperature and a high saline condition. ► The enzyme showed high thermal stability, even incubated in 70 °C for 1 h. ► The enzyme was stable over a broad pH range from 6.0 to 10.0. ► It has a potential in the treatment of heavy metal wastewater for its Pb2+ tolerance. ► The enzyme seemingly has both xylanase activity and cellulase activity.

Keywords
Xylanase; Thermobifida halotolerans YIM 90462T; Thermostable; Extremophiles
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Purification and partial characterisation of a thermostable xylanase from salt-tolerant Thermobifida halotolerans YIM 90462T
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 225–228
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us