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Characterization of leucine amino peptidase from Streptomyces gedanensis and its applications for protein hydrolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
34926 45060 2012 9 PDF Available
Title
Characterization of leucine amino peptidase from Streptomyces gedanensis and its applications for protein hydrolysis
Abstract

The aim of this work was to purify and characterize the extra-cellular leucine amino peptidase (LAP) from Streptomyces gedanensis and also study its applications for protein hydrolysis. The enzyme was purified to homogeneity by ammonium sulfate fractionation and sequential chromatography steps. LAP appeared to be a monomeric enzyme with a molecular weight of ∼75 kDa determined by sodium dodecyl sulfate poly acryl amide gel electrophoresis (SDS-PAGE). The enzyme preferentially hydrolyzed leucine p-nitroanilide followed by Met, Phe, Lys and Arg derivatives. Kinetic studies on the purified enzyme confirmed that it can hydrolyze peptide as well as ester substrates at comparable rates. This amino peptidase was highly resistant to different concentrations of various organic solvents. The characteristics of this amino peptidase, including thermo stability, organic solvent resistance, its activity against various substrates, and also it showed esterase and peptidase activity at comparable rates; identified this amino peptidase as a novel one. The specificity towards aromatic and hydrophobic amino acid residues, the solvent-resistance and thermo stability make this amino peptidase could offer interesting possibilities for various industrial applications including debittering of protein hydrolysates, peptide and ester synthesis.

► Solvent resistant, thermo stable LAP purified from Streptomyces gedanensis. ► LAP showed esterase and peptidase activity at comparable rates. ► It has specificity towards aromatic and hydrophobic amino acid residues. ► Its specificity would offer possibilities for debittering of protein hydrolysates. ► Solvent resistant property gives credence to peptide and ester synthesis.

Keywords
Leucine amino peptidase; Submerged fermentation; Streptomyces gedanensis; Protein hydrolysate; Di-peptide; Peptide hydrolysis
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Characterization of leucine amino peptidase from Streptomyces gedanensis and its applications for protein hydrolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 47, Issue 2, February 2012, Pages 234–242
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us